In clinical and research settings, critical samples such as single-cell cDNA and nucleic acids from assisted reproductive technologies often fall far below the input requirements of traditional nanopore sequencing. While amplification-based library preparation can increase sample quantity, it often leads to uneven genome coverage and reduced accuracy in variant detection—undermining the inherent advantages of nanopore technology. The C1.0 High-Sensitivity Chemistry System introduces an innovative substrate enrichment technology that significantly enhances nucleic acid translocation efficiency per unit time, enabling sequencing with ultralow input as little as picogram levels!