The portable nanopore sequencer, PolyseqOne, was launched by Polyseq Biotech in 2024. Featuring a lightweight design and a single-channel architecture, each independent run utilizes one PolyseqCell chip. It not only offers ultra-long read length, real-time sequencing, direct sequencing, and low-cost advantages, but also stands out for its compact and portable design, ease of operation, and quick experimental setup. This enables rapid deployment for on-site rapid testing and mobile detection scenarios, unlocking broader application potential.